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NDB ID: 6B14    PDB ID: 6B14 


Title:

CRYSTAL STRUCTURE OF SPINACH RNA APTAMER IN COMPLEX WITH FAB BL3-6S97N

Molecular Description:

Heavy chain of Fab BL3-6 S to N mutant
Light chain of Fab BL3-6 S to N mutant/RNA Complex

Structural Keywords:

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 2 protein sequences

Primary Citation:

Koirala, D., Shelke, S.A., Dupont, M., Ruiz, S., DasGupta, S., Bailey, L.J., Benner, S.A., Piccirilli, J.A.
Affinity maturation of a portable Fab-RNA module for chaperone-assisted RNA crystallography. 
Nucleic Acids Res., 46, pp. 2624 - 2635, 2018.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

C 1 2 1

Cell Constants:

a = 146.773   b = 79.993   c = 92.892 (Ångstroms)

α = 90.0   β = 111.76   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.2124 for 119514 reflections in the resolution range 49.54 to 1.64 Ångstroms with Fobs >  1.35 sigma(Fobs) and with I >  0.0 sigma(I)