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NDB ID: 5X2G    PDB ID: 5X2G 


Title:

CRYSTAL STRUCTURE OF CAMPYLOBACTER JEJUNI CAS9 IN COMPLEX WITH SGRNA AND TARGET DNA (AGAAACC PAM)

Molecular Description:

CRISPR-associated endonuclease Cas9/RNA/DNA Complex

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Yamada, M., Watanabe, Y., Gootenberg, J.S., Hirano, H., Ran, F.A., Nakane, T., Ishitani, R., Zhang, F., Nishimasu, H., Nureki, O.
Crystal Structure of the Minimal Cas9 from Campylobacter jejuni Reveals the Molecular Diversity in the CRISPR-Cas9 Systems 
Mol. Cell, 65, pp. 1109 - 1121.e3, 2017.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 21

Cell Constants:

a = 104.345   b = 105.094   c = 136.521 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX (1.11.1_2575: ???) program. The R value is 0.191 for 59303 reflections in the resolution range 52.547 to 2.4 Ångstroms with Fobs >  1.35 sigma(Fobs) and with I >  0.0 sigma(I)