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NDB ID: 5WLH    PDB ID: 5WLH 


Title:

CRYSTAL STRUCTURE OF LBACAS13A H328A (C2C2) BOUND TO PRE-CRRNA (24-NT SPACER)

Molecular Description:

LbaCas13a H328A (C2c2)/RNA Complex

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Knott, G.J., East-Seletsky, A., Cofsky, J.C., Holton, J.M., Charles, E., Doudna, J.A.
Guide bound structures of a divergent RNA-targeting A cleaving CRISPR-Cas13a enzyme 
Nat.Struct.Mol.Biol., , pp. - , 2017.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

C 1 2 1

Cell Constants:

a = 150.842   b = 92.213   c = 115.858 (Ångstroms)

α = 90.0   β = 92.425   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.185843 for 142815 reflections in the resolution range 47.1165 to 1.80001 Ångstroms with Fobs >  1.35144 sigma(Fobs) and with I >  0.0 sigma(I)