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NDB ID: 5W1I    PDB ID: 5W1I 


Title:

CRYSTAL STRUCTURE OF LBACAS13A (C2C2) BOUND TO MATURE CRRNA (20-NT SPACER)

Molecular Description:

LbaCas13a (C2c2)/RNA Complex

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Knott, G.J., East-Seletsky, A., Cofsky, J.C., Holton, J.M., Charles, E., O'Connell, M.R., Doudna, J.A.
Guide-bound structures of an RNA-targeting A-cleaving CRISPR-Cas13a enzyme. 
Nat. Struct. Mol. Biol., 24, pp. 825 - 833, 2017.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1

Cell Constants:

a = 88.78   b = 89.212   c = 116.306 (Ångstroms)

α = 86.14   β = 86.97   γ = 66.16 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.197 for 161242 reflections in the resolution range 48.42 to 2.2 Ångstroms with Fobs >  1.959 sigma(Fobs) and with I >  0.0 sigma(I)