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NDB ID: 5VW1    PDB ID: 5VW1 


Title:

CRYSTAL STRUCTURE OF SPYCAS9-SGRNA-ACRIIA4 TERNARY COMPLEX

Molecular Description:

CRISPR-associated endonuclease Cas9/Csn1
anti-CRISPR protein AcrIIA4/RNA Complex

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 2 protein sequences

Primary Citation:

Yang, H., Patel, D.J.
Inhibition Mechanism of an Anti-CRISPR Suppressor AcrIIA4 Targeting SpyCas9. 
Mol. Cell, 67, pp. 117 - 127.e5, 2017.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 2 21 21

Cell Constants:

a = 72.194   b = 101.242   c = 303.563 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.1805 for 65587 reflections in the resolution range 48.021 to 2.598 Ångstroms with Fobs >  1.35 sigma(Fobs) and with I >  0.0 sigma(I)