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NDB ID: 5VI8    PDB ID: 5VI8 


Title:

STRUCTURE OF A MYCOBACTERIUM SMEGMATIS TRANSCRIPTION INITIATION COMPLEX WITH AN UPSTREAM-FORK PROMOTER FRAGMENT

Molecular Description:

RNA polymerase-binding protein RbpA
DNA-directed RNA polymerase subunit alpha
DNA-directed RNA polymerase subunit beta
DNA-directed RNA polymerase subunit beta'
DNA-directed RNA polymerase subunit omega
RNA polymerase sigma factor SigA/DNA Complex

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 7 protein sequences

Primary Citation:

Hubin, E.A., Fay, A., Xu, C., Bean, J.M., Saecker, R.M., Glickman, M.S., Darst, S.A., Campbell, E.A.
Structure and function of the mycobacterial transcription initiation complex with the essential regulator RbpA. 
Elife, 6, pp. - , 2017.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 133.012   b = 161.633   c = 139.211 (Ångstroms)

α = 90.0   β = 107.72   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX (1.10.1_2155: ???) program. The R value is 0.2409 for 143074 reflections in the resolution range 51.99 to 2.76 Ångstroms with Fobs >  1.33 sigma(Fobs) and with I >  0.0 sigma(I)