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NDB ID: 5V0Q    PDB ID: 5V0Q 


Title:

ORIGINAL ENGINEERED VARIANT OF I-ONUI MEGANUCLEASE TARGETING THE HIV INTEGRASE GENE; HARBORS 49 POINT MUTATIONS RELATIVE TO WILD-TYPE I-ONUI

Molecular Description:

I-OnuI_e-vHIVInt_v1/DNA Complex

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Niyonzima, N., Lambert, A.R., Werther, R., De Silva Feelixge, H., Roychoudhury, P., Greninger, A.L., Stone, D., Stoddard, B.L., Jerome, K.R.
Tuning DNA binding affinity and cleavage specificity of an engineered gene-targeting nuclease via surface display, flow cytometry and cellular analyses. 
Protein Eng. Des. Sel., 30, pp. 503 - 522, 2017.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 21

Cell Constants:

a = 39.693   b = 74.419   c = 164.861 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.1901 for 19890 reflections in the resolution range 44.207 to 2.4 Ångstroms with Fobs >  1.35 sigma(Fobs) and with I >  0.0 sigma(I)