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NDB ID: 5TJG    PDB ID: 5TJG 


Title:

THERMUS AQUATICUS DELTA1.1-SIGMAA HOLOENZYME/DOWNSTREAM-FORK PROMOTER COMPLEX WITH AN OPEN CLAMP

Molecular Description:

DNA-directed RNA polymerase subunit alpha
DNA-directed RNA polymerase subunit beta
DNA-directed RNA polymerase subunit beta'
DNA-directed RNA polymerase subunit omega
RNA polymerase sigma factor SigA/DNA Complex

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 5 protein sequences

Primary Citation:

Feklistov, A., Bae, B., Hauver, J., Lass-Napiorkowska, A., Kalesse, M., Glaus, F., Altmann, K.H., Heyduk, T., Landick, R., Darst, S.A.
RNA polymerase motions during promoter melting. 
Science, 356, pp. 863 - 866, 2017.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1

Cell Constants:

a = 104.098   b = 111.423   c = 143.177 (Ångstroms)

α = 104.12   β = 99.66   γ = 109.43 (degrees)

Refinement:

The structure was refined using the PHENIX (1.10.1_2155: ???) program. The R value is 0.226 for 169540 reflections in the resolution range 38.627 to 2.6 Ångstroms with Fobs >  1.96 sigma(Fobs) and with I >  0.0 sigma(I)