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NDB ID: 5THG    PDB ID: 5THG 


Title:

ENGINEERED VARIANT OF I-ONUI MEGANUCLEASE TARGETING THE HIV CCR5 GENE; HARBORS 43 POINT MUTATIONS RELATIVE TO WILD-TYPE I-ONUI

Molecular Description:

I-OnuI_e-hCCR5/DNA Complex

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Werther, R., Hallinan, J.P., Lambert, A., Haven, K., Jarjor, J., Stoddard, B.L.
The structural basis of altered gene specificity resulting from meganuclease and MegaTAL engineering 
to be published, , pp. - , 0.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 21

Cell Constants:

a = 75.959   b = 112.351   c = 122.879 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.2264 for 19313 reflections in the resolution range 47.769 to 3.106 Ångstroms with Fobs >  1.35 sigma(Fobs) and with I >  0.0 sigma(I)