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NDB ID: 5T3K    PDB ID: 5T3K 


Title:

FLUORESCENCE DETECTION OF RNA-LIGAND BINDING AND CRYSTAL STRUCTURE DETERMINATION OF RIBOSOMAL DECODING SITE RNA USING A HEAVY ATOM CONTAINING FLUORESCENT RIBONUCLEOSIDE

Molecular Description:

RNA (5'-R(*GP*GP*UP*GP*GP*UP*GP*AP*AP*GP*UP*CP*GP*CP*UP*GP*G)-3')
RNA (5'-R(*CP*AP*GP*CP*GP*(U5S)P*CP*AP*CP*AP*CP*CP*AP*CP*CP*C)-3')

Structural Keywords:

A DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

No Protein Sequence Found

Primary Citation:

Nuthanakanti, A., Boerneke, M.A., Hermann, T., Srivatsan, S.G.
Structure of the Ribosomal RNA Decoding Site Containing a Selenium-Modified Responsive Fluorescent Ribonucleoside Probe. 
Angew. Chem. Int. Ed. Engl., 56, pp. 2640 - 2644, 2017.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 31.553   b = 86.956   c = 32.564 (Ångstroms)

α = 90.0   β = 94.14   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.2028 for 7483 reflections in the resolution range 26.02 to 2.142 Ångstroms with Fobs >  1.35 sigma(Fobs) and with I >  0.0 sigma(I)