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NDB ID: 5ON3    PDB ID: 5ON3 


Title:

QUATERNARY COMPLEX OF MUTANT T252A OF E. COLI LEUCYL-TRNA SYNTHETASE WITH TRNA(LEU), LEUCYL-ADENYLATE ANALOGUE, AND POST-TRANSFER EDITING ANALOGUE OF LEUCINE IN THE AMINOACYLATION CONFORMATION

Molecular Description:

Leucine--tRNA ligase/RNA Complex

Structural Keywords:

A DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Dulic, M., Cvetesic, N., Zivkovic, I., Palencia, A., Cusack, S., Bertosa, B., Gruic-Sovulj, I.
Kinetic Origin of Substrate Specificity in Post-Transfer Editing by Leucyl-tRNA Synthetase. 
J. Mol. Biol., 430, pp. 1 - 16, 2018.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

C 1 2 1

Cell Constants:

a = 159.0   b = 68.7   c = 227.2 (Ångstroms)

α = 90.0   β = 102.8   γ = 90.0 (degrees)

Refinement:

The structure was refined using the REFMAC program. The R value is 0.24293 for 41189 reflections in the resolution range 221.55 to 3.1 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)