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NDB ID: 5NFV    PDB ID: 5NFV 


Title:

CRYSTAL STRUCTURE OF CATALYTICALLY INACTIVE FNCAS12 MUTANT BOUND TO AN R-LOOP STRUCTURE CONTAINING A PRE-CRRNA MIMIC AND FULL-LENGTH DNA TARGET

Molecular Description:

CRISPR-associated endonuclease Cpf1/DNA Complex

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Swarts, D.C., van der Oost, J., Jinek, M.
Structural Basis for Guide RNA Processing and Seed-Dependent DNA Targeting by CRISPR-Cas12a. 
Mol. Cell, 66, pp. 221 - 233.e4, 2017.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 82.421   b = 143.703   c = 84.628 (Ångstroms)

α = 90.0   β = 94.16   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.207 for 131823 reflections in the resolution range 46.568 to 2.501 Ångstroms with Fobs >  1.37 sigma(Fobs) and with I >  0.0 sigma(I)