******************************************************************************

******************************************************************************

******************************************************************************
April 15, 1999: Structures can be deposited to the NDB using the
		AutoDep Input Tool, a Web-based tool developed by the RCSB.
		ADIT is available at http://pdb.rutgers.edu/adit/.
******************************************************************************

******************************************************************************

******************************************************************************


	     Deposition Form for Nucleic Acid Crystal Structures

To deposit data for a nucleic acid crystal structure, complete the following
deposition form as completely as possible.  The form is a shortened version of
the PDB deposition form (Version 3.40) and does not include the items SSBOND,
SITE, HELIX, SHEET, and TURN.

Send this form, along with the coordinate file, to
deposit@ndbserver.rutgers.edu.  Structure factors, if submitted, may be
submitted in any format. The paper manuscript should also be sent via
facsimile or air mail.

Questions and manuscripts should be sent to:

         deposit@ndbserver.rutgers.edu 

         The Nucleic Acid Database
         Rutgers, the State University
         Wright-Reiman Laboratories
         Chemistry
         610 Taylor Road
         Piscataway, NJ 08854-8087
         Phone: 732-445-0103 
         Fax: 732-445-4320 

******************************************************************************

CONTACT INFORMATION 

Primary Contact

The primary contact has authority to grant approval for release of the
completed entry. The prepared entry, validation diagnostics, and
approval request will be sent to the primary contact. Typically this
is the Principal Investigator.

First name: 
Middle name or initial: 
Family name: 

e-mail address: 

Department and institution: 

Mailing address: 

Fax number: 
Telephone number: 

Secondary Contact

First name: 
Middle name or initial: 
Family name: 

e-mail address: 

Department and institution: 

Mailing address: 

Fax number: 
Telephone number: 

--------------------------------------------------------------------------------
--------------------------------------------------------------------------------

AUTHORS, TITLE AND REFERENCE(S)

Please, specify authors and title(s) of this entry. 

Cite both the primary reference which identifies the deposited set of
coordinates as well as other important references. Repeat block as many times
as necessary.

Is this the primary citation reporting this structure? 
    [ ] No 
    [ ] Yes 

Author(s):

Journal or book name:

Year:

Volume number:

Page numbers (first-last):

Article or chapter title:

Thesis title:

Publisher, for non-journals:

Editor(s), for non-journals:

Place of publication, for non-journals:

Series info:
Other info: 


--------------------------------------------------------------------------------
--------------------------------------------------------------------------------

CRYSTAL GROWTH

Give the crystal's solvent content and crystallization conditions. 

Crystallization method (hanging/sitting drop...): 

pH of crystallization: 
Temperature of crystallization: 

Percent solvent content (Vs): 
Matthews coefficient (Vm): 

Crystallization conditions: 


--------------------------------------------------------------------------------
--------------------------------------------------------------------------------

CRYSTAL DATA

Unit-cell data and coordinate system information. All PDB coordinate entries
are released in an orthogonal coordinate frame, in Angstroms. All submissions
must have this section completed.

Unit Cell and Crystal Data

Provide the unit cell information for your crystal. 

a (Angstroms): 
b (Angstroms): 
c (Angstroms): 
alpha (degrees): 
beta (degrees): 
gamma (degrees): 

space group: 

Is this a non-standard space group or setting? 
        [ ] No 
        [ ] Yes 

Z-value: 

If this is a non-standard space group or setting, choose Yes above, and
provide the symmetry operators here.

Symmetry operators for non-standard space group or setting, 
separated by semicolons: 

Explain any unusual unit cell data: 


--------------------------------------------------------------------------------
--------------------------------------------------------------------------------

EXPERIMENTAL DETAILS 


[ ] X-ray diffraction 
    type of radiation used 
        [ ] monochromatic 
        [ ] laue 

    sample type 
        [ ] single crystal 
        [ ] fiber 
        [ ] polycrystalline fiber 

Number of crystals used in the experiment: 


X-RAY SOURCE

if laboratory source: x-ray generator type (e.g. rotating anode) and model:

if synchrotron: name of synchrotron and beamline
        [ ] APS 
        [ ] CHESS 
        [ ] DESY-EMBL,HAMBURG 
        [ ] ESRF 
        [ ] LURE 
        [ ] NSLS 
        [ ] PHOTON FACTORY 
        [ ] SRS 
        [ ] SSRL 
        [ ] OTHER (specify beamline)

Monochromator: 

Optics: 

Detector type (e.g. image plate): 

Detector manufacturer: 

Date of data collection: 

Temperature of experiment, in Kelvin: 

Wavelength(s) or wavelength range (A): 

--------------------------------------------------------------------------------
--------------------------------------------------------------------------------

DATA COLLECTION

Total number of unique reflections collected: 

Number of unique reflections collected with I or F higher than the 
specified rejection limit: 

Rejection criteria (sigma(I)):
Rejection criteria (sigma(F)):

Completeness for range (%): 

Resolution range high (A): 
Resolution range low (A): 

Merging R value (I): 
Data redundancy: 

--------------------------------------------------------------------------------

Information on the highest Resolution Shell

Resolution range high (A): 
Resolution range low (A): 

Percent possible reflections (%): 

Merging R value (I): 
Data redundancy: 

Total number of unique reflections collected in the shell: 

--------------------------------------------------------------------------------
--------------------------------------------------------------------------------

COMPOUND AND SOURCE 

Please, describe the molecular contents of the entry, including the 
macromolecules and their biological sources.

Keyword(s) describing the entry: 


Name and describe each unique macromolecule, repeating the block for each
molecule of a macromolecular complex.

Molecule name: 

Chain name(s): 

Domain or fragment: 

Synonyms: 

Mutations: 

Biological unit: 

Compound, other details:

--------------------------------------------------------------------------------

Please, describe the biological source. 

Scientific name of organism: 

Common name of organism: 

Source, other details: 

Was the molecule chemically synthesized? 
    [ ] No 
    [ ] Yes 

    optional remark describing the synthetic sequence: 

Was the molecule made using recombinant techniques? 
    [ ] No 
    [ ] Yes 

If this is an engineered molecule, also describe the experiment. 

Expression system organism: 
Expression system strain:
Expression system plasmid: 
Expression system organelle: 

Optional comment on the experiment: 


--------------------------------------------------------------------------------

SEQUENCE 

Describe any non-standard residues. These may have arisen due to
post-translational or chemical modifications. Repeat block as many
times as necessary.

1) MODIFIED RESIDUES

Non-standard residue name, as used in the submitted coordinates: 

Standard residue name: 

Description of the modification: 


--------------------------------------------------------------------------------

2) HETEROGENS 

Are there any non-water hetero atoms included in this structure? 
    [ ] No 
    [ ] Yes 

If yes, please, describe prosthetic groups, inhibitors, solvents
(except water), and/or ions. Repeat this block for each unique HET
group.

HET residue name used in this deposition: 

HET group trivial name: 

HET group systematic name: 

Synonyms for HET group: 

Empirical formula: 

Charge: 

Other details: 


--------------------------------------------------------------------------------
--------------------------------------------------------------------------------

SOFTWARE USED

Intensity-integration software: 

Data scaling software: 

Structure solution program: 

Details: 

--------------------------------------------------------------------------------
--------------------------------------------------------------------------------

REFINEMENT 


Quality of fit and data used for refinement

R value (all reflections): 
R value (working + test set): 
R value (working set): 

Resolution range high (Angstroms): 
Resolution range low (Angstroms): 

Number of observed reflections: 
Completeness for range (%): 
Total number of reflections (no cutoff): 
Data cutoff (sigma(F)): 
Data cutoff (sigma(I)): 


Cross Validation

Free R value: 
Free R value test set count: 
Free R value test set size (%): 
Free R value test set selection: 


RMS deviations from ideal values 

Bond lengths (A): 
Bond angles (degrees): 
Bond angles (A): 


Number of non-hydrogen refined atoms

Protein atoms: 
Nucleic acid atoms: 
Heterogen atoms: 
Solvent atoms: 

--------------------------------------------------------------------------------

REFINEMENT PROGRAM

NOTE:	If you have a complete X-PLOR, CNS or SHELXL-97 output file in PDB
	format, you may send this file and ignore the remaining part of 
	this deposition form.


[ ] X-PLOR 
Version of X-PLOR used for the refinement: 

[ ] CNS 
Version of CNS used for the refinement: 

[ ] TNT 
Version of TNT used for the refinement: 

[ ] PROLSQ and related 
Indicate which program/extension was used 
        [ ] PROLSQ 
        [ ] CCP4 
        [ ] PROFFT 
        [ ] REFMAC 
        [ ] GPRLSA 
        [ ] Other (please, specify program name and authors): 

[ ] SHELX 
Version of SHELX used for the refinement: 

[ ] NUCLSQ 

[ ] Other 
Refinement program name: 
Refinement program authors: 

--------------------------------------------------------------------------------